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Title   융모상피암 세포주에서 Transforming Growth Fasctor-β1 의 세포 성장 및 c-myc 암유전자 발현 억제효과 ( Inhibitory Effect of Transforming Growth Fasctor-β1 on Cellular Proliferation and c-myc Expression in Choriocarcinoma Cell Line )
Publicationinfo   1995 Jan; 027(06): 961-968.
Key_word   Transforming growth factor-￥a1, c-myc oncogene, Choriocarcinoma
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Abstract   Modulation of cellular proliferation and c-myc oncogene expression after treatment of human choriocarcinoma cell line(BeWo) with trsnsorming growth factor-￥a1(TGF-￥a1) have been investigated by MTT [3-(4,5-dimethylthiaxol-2-yl)-2, 5-diphenyltetrazolium bromide] assay and Northern blotting analysis, respectively. TGF-￥a1 inhibited BeWo cell growth in a dose dependent manner at concentrations of 0.00110 ng/mL. When BeWo cells were exposed to 10 ng/ml of TGF-￥a1,c-myc was repressed after 4hr and completely aboiished at 24hr of stimulation. These results show that TGF-￥a1 may play an antiproliferative effect on BeWo cell growth via c-myc gene product. In vitro analysis of cellular and molecular alterations in BeWo cells in this study may aid in elucidating the mechanism of action of TGF-￥a1 on human choriocarcinoma tissues in vivo.
저 자   김사진(Sa Jin Kim),김진우(Jin Woo Kim),나종구(Jong Gu Rha),이귀세라(Guisera Lee),송승규(Seung Kyu Song),남궁성은(Sung Eun Namkoong)